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dig rna labeling kit  (Millipore)


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    Structured Review

    Millipore dig rna labeling kit
    Dig Rna Labeling Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dig+rna+labeling+kit/dig+rna+labeling+kit/pm40595562-314-12-16
    Average 90 stars, based on 1 article reviews
    dig rna labeling kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Construct:

    Article Title: microRNA-1 regulates sea urchin skeletogenesis by directly targeting skeletogenic genes and modulating components of signaling pathways.
    Article Snippet: microRNAs are evolutionarily conserved non-coding RNAs that direct post-transcriptional regulation of target transcripts.. In vertebrates, microRNA-1 (miR-1) is expressed in muscle and has been found to play critical regulatory roles in vertebrate angiogenesis, a process that has been proposed to be analogous to sea urchin skeletogenesis.. Results indicate that both miR-1 inhibitor and miR-1 mimic-injected larvae have significantly less F-actin enriched circumpharyngeal muscle fibers and fewer gut contractions.

    In Vitro:

    Article Title: microRNA-1 regulates sea urchin skeletogenesis by directly targeting skeletogenic genes and modulating components of signaling pathways.
    Article Snippet: microRNAs are evolutionarily conserved non-coding RNAs that direct post-transcriptional regulation of target transcripts.. In vertebrates, microRNA-1 (miR-1) is expressed in muscle and has been found to play critical regulatory roles in vertebrate angiogenesis, a process that has been proposed to be analogous to sea urchin skeletogenesis.. Results indicate that both miR-1 inhibitor and miR-1 mimic-injected larvae have significantly less F-actin enriched circumpharyngeal muscle fibers and fewer gut contractions.

    Article Title: Self-formation of concentric zones of telencephalic and ocular tissues and directional retinal ganglion cell axons
    Article Snippet: .. DIG- labeled anti- sense RNA probes for in situ hybridization were generated via in vitro transcription using a DIG RNA labeling kit (Millipore Sigma- Aldrich 11175025910). ..

    Article Title: Self-formation of concentric zones of telencephalic and ocular tissues and directional retinal ganglion cell axons
    Article Snippet: .. DIG-labeled anti-sense RNA probes for in situ hybridization were generated via in vitro transcription using a DIG RNA labeling kit (Millipore Sigma-Aldrich 11175025910). ..

    Labeling:

    Article Title: microRNA-1 regulates sea urchin skeletogenesis by directly targeting skeletogenic genes and modulating components of signaling pathways.
    Article Snippet: microRNAs are evolutionarily conserved non-coding RNAs that direct post-transcriptional regulation of target transcripts.. In vertebrates, microRNA-1 (miR-1) is expressed in muscle and has been found to play critical regulatory roles in vertebrate angiogenesis, a process that has been proposed to be analogous to sea urchin skeletogenesis.. Results indicate that both miR-1 inhibitor and miR-1 mimic-injected larvae have significantly less F-actin enriched circumpharyngeal muscle fibers and fewer gut contractions.

    Article Title: Self-formation of concentric zones of telencephalic and ocular tissues and directional retinal ganglion cell axons
    Article Snippet: .. DIG- labeled anti- sense RNA probes for in situ hybridization were generated via in vitro transcription using a DIG RNA labeling kit (Millipore Sigma- Aldrich 11175025910). ..

    Article Title: Sin3a associated protein 130 kDa, sap130, plays an evolutionary conserved role in zebrafish heart development
    Article Snippet: .. RNA probe generation and whole mount in situ hybridization for nkx2.5 , ltbp3 , myh7 and myh6 was performed as previously described with DIG RNA labeling kit (Millipore Sigma cat# 11175025910) ( ). .. Total RNA was extracted from whole embryos or isolated hearts (36hpf and 48hpf, respectively) using Trizol (Invitrogen) and was purified with the RNeasy Micro Kit (Qiagen#74004).

    Article Title: Microenvironmental control of hematopoietic stem cell fate via CXCL8 and protein kinase C
    Article Snippet: In vitro transcription was performed using the Maxiscript T7 transcription kit (Thermo Fisher AM1314). .. DIG-labeled probe was generated using the Roche DIG RNA labeling kit (Sigma-Aldrich 11175025910) and resuspended in Hyb+ at a concentration of 50 ng/μL of RNA probe. ..

    Article Title: p65 signaling dynamics drive the developmental progression of hematopoietic stem and progenitor cells through cell cycle regulation
    Article Snippet: .. Probes used for cmyb , runx1, mpx, mpeg, l-plastin gata1 , and kdrl transcripts were generated from linearized plasmids using the DIG RNA Labeling Kit (MilliporeSigma; 11175025910). .. Embryos were imaged using a Leica M165FC stereomicroscope equipped with a DFC295 color digital camera (Leica) and FireCam software (Leica) with Leica Application Suite X (v3.7.0.20979).

    Article Title: Self-formation of concentric zones of telencephalic and ocular tissues and directional retinal ganglion cell axons
    Article Snippet: .. DIG-labeled anti-sense RNA probes for in situ hybridization were generated via in vitro transcription using a DIG RNA labeling kit (Millipore Sigma-Aldrich 11175025910). ..

    In Situ Hybridization:

    Article Title: Self-formation of concentric zones of telencephalic and ocular tissues and directional retinal ganglion cell axons
    Article Snippet: .. DIG- labeled anti- sense RNA probes for in situ hybridization were generated via in vitro transcription using a DIG RNA labeling kit (Millipore Sigma- Aldrich 11175025910). ..

    Article Title: Sin3a associated protein 130 kDa, sap130, plays an evolutionary conserved role in zebrafish heart development
    Article Snippet: .. RNA probe generation and whole mount in situ hybridization for nkx2.5 , ltbp3 , myh7 and myh6 was performed as previously described with DIG RNA labeling kit (Millipore Sigma cat# 11175025910) ( ). .. Total RNA was extracted from whole embryos or isolated hearts (36hpf and 48hpf, respectively) using Trizol (Invitrogen) and was purified with the RNeasy Micro Kit (Qiagen#74004).

    Article Title: Self-formation of concentric zones of telencephalic and ocular tissues and directional retinal ganglion cell axons
    Article Snippet: .. DIG-labeled anti-sense RNA probes for in situ hybridization were generated via in vitro transcription using a DIG RNA labeling kit (Millipore Sigma-Aldrich 11175025910). ..

    Generated:

    Article Title: Self-formation of concentric zones of telencephalic and ocular tissues and directional retinal ganglion cell axons
    Article Snippet: .. DIG- labeled anti- sense RNA probes for in situ hybridization were generated via in vitro transcription using a DIG RNA labeling kit (Millipore Sigma- Aldrich 11175025910). ..

    Article Title: Microenvironmental control of hematopoietic stem cell fate via CXCL8 and protein kinase C
    Article Snippet: In vitro transcription was performed using the Maxiscript T7 transcription kit (Thermo Fisher AM1314). .. DIG-labeled probe was generated using the Roche DIG RNA labeling kit (Sigma-Aldrich 11175025910) and resuspended in Hyb+ at a concentration of 50 ng/μL of RNA probe. ..

    Article Title: p65 signaling dynamics drive the developmental progression of hematopoietic stem and progenitor cells through cell cycle regulation
    Article Snippet: .. Probes used for cmyb , runx1, mpx, mpeg, l-plastin gata1 , and kdrl transcripts were generated from linearized plasmids using the DIG RNA Labeling Kit (MilliporeSigma; 11175025910). .. Embryos were imaged using a Leica M165FC stereomicroscope equipped with a DFC295 color digital camera (Leica) and FireCam software (Leica) with Leica Application Suite X (v3.7.0.20979).

    Article Title: Self-formation of concentric zones of telencephalic and ocular tissues and directional retinal ganglion cell axons
    Article Snippet: .. DIG-labeled anti-sense RNA probes for in situ hybridization were generated via in vitro transcription using a DIG RNA labeling kit (Millipore Sigma-Aldrich 11175025910). ..

    Concentration Assay:

    Article Title: Microenvironmental control of hematopoietic stem cell fate via CXCL8 and protein kinase C
    Article Snippet: In vitro transcription was performed using the Maxiscript T7 transcription kit (Thermo Fisher AM1314). .. DIG-labeled probe was generated using the Roche DIG RNA labeling kit (Sigma-Aldrich 11175025910) and resuspended in Hyb+ at a concentration of 50 ng/μL of RNA probe. ..

    other:

    Article Title: RFC1 regulates the expansion of neural progenitors in the developing zebrafish cerebellum.
    Article Snippet: Larvae were incubated in primary antibodies: anti-pvalb7 (gift fromM.Hibi; 1:1000), anti-neurod1 (gift from M. Hibi; 1:500), anti-vglut1 (gift from M. Hibi; 1:1000), antigH2AX (GTX127342, GenTex; 1:1000), anti-PH3 (Millipore 09838; 1:500), or anti-cleaved caspase-3 (9661, Cell Signaling; 1:200).

    Article Title: RFC1 regulates the expansion of neural progenitors in the developing zebrafish cerebellum
    Article Snippet: The following day, after several washes in PBS1X-Tween 0.1% (P5927, Sigma), larvae were incubated with a secondary antibody diluted in blocking solution: goat anti-mouse Alexa Fluor 488 (A11029, Millipore Sigma; 1:500), goat anti-rabbit Alexa Fluor 488 (A11034, Millipore Sigma; 1:500).



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